Speaker Bio

Dr. Zhaochao Xu received his PhD in 2006 from Dalian University of Technology under the supervision of Prof. Xuhong Qian. Subsequently, he joined the group of Juyoung Yoon at Ewha Womans University as a postdoctoral researcher. Since October 2008, he was a Herchel Smith Research Fellow at University of Cambridge in David Spring’s group. In 2011, he moved to Dalian Institute of Chemical Physics, CAS, where he is currently a Professor. His research is focused on the development of new fluorescent dyes to explore biology. His group is discovering and revealing new fluorescence mechanisms and the structural and environmental dependence of absorption and fluorescence, which can be applied in the study of interaction between fluorescent dyes and biomolecules. Through the thorough understanding of the structure-fluorescence relationship of dyes, the group is developing new dyes for single-molecule and super-resolution fluorescence imaging, with the required time resolution and long-term dynamic imaging capability. He has published more than 130 papers with more than 11000 citations, his h-index is 48.

中科院大连化学物理研究所研究员，博士生导师。研究聚焦在发展新型生物功能荧光染料与化学生物学研究，包括新型荧光染料设计和发光构效关系研究，蛋白标记和识别，超分辨荧光成像，和荧光成像在化工过程和医学引导手术应用等。发表论文130余篇，被引用11000多次，h指数48。获得国家基金委杰出青年基金、国家领军人才、创新人才推进计划中青年科技创新领军人才、基金委优秀青年基金、中科院百人计划等项目资助。2015-2021年连续入选爱思唯尔中国高被引学者榜单，获得MSMLG Czarnik Emerging Investigator Awards。学术兼职包括：Coord. Chem. Rev. 编委，Chin. Chem. Lett. 副主编，华东理工大学学报执行编委，中国分析测试协会青年学术委员会委员，中科院青年联合会第四届委员，辽宁省细胞生物学学会肿瘤细胞与分子生物学分会常务理事。

Abstract

Super-resolution fluorescence imaging visualizes biomolecules at the nanoscale, but breakthroughs in spatiotemporal resolution and dynamic imaging are challenges. The switchable fluorescent dyes break through the diffraction limit by exchanging time for space and realizes super-resolution imaging. However, the brightness, photostability, and switch regulation of fluorescent dyes are limited, and the current spatial and temporal resolution is low, making it difficult to process imaging. In recent years, based on the systematic research and innovation of the dye luminescence theory, the reporter developed "homogeneous scintillation fluorescent dyes regulated by intramolecular hydrogen bonds", and proposed a "buffering fluorescent probe" strategy to resist photobleaching, and realized the continuous super-resolution imaging photobleaching at 9 nm / 20 ms spatio-temporal resolution for 40 minutes. A variety of unreported organelle dynamic behaviors and modes of action were discovered, revealing the flow, exchange and interaction mechanisms between substances.

超分辨荧光成像在纳米尺度可视化生物分子，但时空分辨率的突破和动态成像是挑战。荧光染料光开关以时间换空间突破衍射极限，实现超分辨成像。但荧光染料亮度、光稳定性和开关调控受限等，目前时空分辨率低，难以过程成像。报告人近年来基于染料发光理论的系统研究和创新，发展“分子内氢键调控的匀质闪烁荧光染料”，提出了“缓冲荧光探针”抵抗光漂白的策略，实现了在9 nm / 20 ms时空分辨率下连续超分辨成像40分钟；发现了多种未见报道的细胞器动态行为和作用方式，揭示物质间流动、交换和作用机制；发展超分辨成像染料高效开发方法，完备的荧光试剂应用于不同类型超分辨成像显微镜。